Cellulose Acetate Membrane Electrophoresis

Kini Cellulose Acetate Membrane Electrophoresis?

Cellulose Acetate Membrane Electrophoresis jẹ ọkan iru awọn ilana elekitirophoresis eyiti o nlo awọ sẹẹli acetate cellulose bi media atilẹyin fun awọn idanwo.

Cellulose acetate jẹ iru acetate ti cellulose ti o jẹ acetylated lati cellulose's hydroxyl. Nigbati o ba jẹ tiotuka ni ojutu Organic gẹgẹbi acetone, o le jẹ ti a bo si isokan ati fiimu ti o dara pẹlu awọn pores micro. Iwọn fiimu naa wa ni ayika 0.1-0.15mm ti o dara fun idanwo.

Awọn anfani ti cellulose acetate awo

Ni afiwe pẹlu iwe fliter, o ni awọn anfani wọnyi:

1.O dara ipa ti Iyapa. Fun awọn ayẹwo amuaradagba, cellulose acetate membrane fa diẹ ninu wọn, lẹhin awọ, o le decolorize patapata lati awọ abẹlẹ. Awọn ẹgbẹ awọ jẹ kedere pupọ, nitorinaa o ṣe agbega deede fun ipinnu ipinnu pipo.
2.Fast ati fi akoko pamọ. Membrane acetate cellulose ko kere si hydyophilic ju iwe fliter lọ, nitorinaa iṣẹ elekitiro-osmosis ko kere ju iwe fliter, ati lọwọlọwọ ina ni o waiye nipasẹ awọn apẹẹrẹ ti o le kuru akoko ipinya ati dinku akoko electrophoresis. Ni deede, akoko cellulose acetate membrane electrophoresis jẹ nipa awọn iṣẹju 45-60. Gbogbo ilana ti electrophoresis pẹlu dyeing ati decoloration nikan nilo awọn iṣẹju 90.
3.High ifamọ ati ki o kere awọn ayẹwo lati ṣee lo. O nilo nikan 2 μL omi ara ni serum protein electrophoresis.
4.Wide elo. Diẹ ninu awọn amuaradagba ko rọrun lati ya sọtọ lori iwe electrophoresis, bii Alpha-fetoprotein, enzymu bacteriolytic, insulin, ati histone bbl Ṣugbọn o dara julọ lati lo cellulose acetate membrane electrophoresis lati ya wọn sọtọ.
5.Easy lati tọju ati quantification. Lẹhin ti dyeing cellulose acetate membrane electrophoresis awọn ayẹwo, immerse awọn ayẹwo ni ojutu adalu ti glacial acetic acid ati ethanol lati ṣe kan sihin gbẹ awo ti o wa ni fipamọ fun igba pipẹ.

Nitori cellulose acetate membrane electrophoresis jẹ rọrun lati ṣiṣẹ, ni bayi o ti wa ni lilo pupọ ni itupalẹ ati idanwo amuaradagba pilasima, lipoprotein, glycoprotein, Alpha-fetoprotein, enzymu, polypeptide, acid nucleic ati biomacromolecule miiran.

Igbaradi fun Cellulose Acetate Membrane Electrophoresis

Ohun elo ti a lo pẹlu ipese agbara foliteji kekere, ojò electrophoresis petele kan. Awọn media ti o ṣe atilẹyin jẹ awo awọ acetate cellulose.

Awọn reagents:

1.pH8.6 barbitol buffer ojutu: Diethyl barbituric acid, Diethyl sodium pentobarbital;
2.Stain: Ponceau S, Trichloroacetic;
3.TBS / T tabi PBS / T: 95% ethanol, glacial acetic acid;
4.Cleaning Solusan: ethanol anhydrous, glacial acetic acid.

Beijing Liuyi Biotechnology Co., ltd ni eto ti o dagba fun cellulose acetate membrane electrophoresis. Awoṣe DYCP-38C jẹ iyẹwu elege fun iwe ati ifaworanhan electrophoresis, ati cellulose acetate membrane electrophoresis. Ara akọkọ jẹ apẹrẹ, ko si iṣẹlẹ jijo. Awọn awoṣe ipese agbara DYY-2C, DYY-6C, DYY-6D, DYY-8C , DYY-10C ati DYY-12 le pese agbara fun DYCP-38C. Ni gbogbogbo, alabara fẹran lati yan awoṣe DYY-6C bi ipese agbara fun DYCP-38C.

Gẹgẹbi ọja pataki fun DYCP-38C ojò electrophoresis, Liuyi tun pese awo awọ acetate cellulose. Awọn iwọn ti o wọpọ mẹta ti awọ ara ilu, 7 * 9cm, 2 * 8cm ati 12 * 8cm, ati pe a le pese iwọn adani ti awo sẹẹli acetate cellulose gẹgẹbi awọn ibeere rẹ daradara.

Awọn akọsilẹ nipa idanwo:

1. Load awọn ayẹwo lori ẹgbẹ ti o ni inira: 1-2UL jẹ o dara
2.Constant lọwọlọwọ electrophoresis: agbara lọwọlọwọ 0.4 ~ 0.6m A / cm
3.pH8.6 barbitol ifipamọ ojutu: Ionic agbara 0.06
4.Dyeing akoko: 5 iṣẹju to
5.Preservation: fi maapu elecrtophoresis ti o gbẹ ni ojutu mimọ fun awọn iṣẹju 10-15, lẹhinna mu jade ki o fi sii lori gilasi mimọ, lẹhin ti o gbẹ, yoo di maapu fiimu ti o han gbangba.

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